Supplementary Materials

The PDF file includes:

  • Fig. S1. Evaluation of the effect of the HaloTag fusion to mGluR3.
  • Fig. S2. Comparison of HaloTag and SNAP-tag ligands by single-molecule imaging.
  • Fig. S3. Example of the VB-HMM analysis of mGluR3 trajectories.
  • Fig. S4. Dose-dependent change in the time constants of the state transition.
  • Fig. S5. Dose-dependent change in the diffusion coefficient of each diffusion state.
  • Fig. S6. Correlation between mean oligomer size and receptor density under various ligand conditions.
  • Fig. S7. Correlation between DAv and receptor density under various ligand conditions.
  • Fig. S8. MSD-Δt plots of the trajectories of GPCRs with or without agonist.
  • Fig. S9. Histograms showing displacement during 33 ms of the trajectories for mGluR3, Go protein, and CLC molecules, categorized into four diffusion states, using VB-HMM analysis.
  • Legends for Movies S1 to S5

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). TIRFM of TMR-labeled mGluR3 molecules on a HEK 293 cell.
  • Movie S2 (.mp4 format). Dual-color TIRFM of TMR-labeled mGluR3 and SiR-labeled Go protein.
  • Movie S3 (.mp4 format). Accumulation of mGluR3 molecules followed by disappearance with rapid movement.
  • Movie S4 (.mp4 format). Dual-color TIRFM of TMR-labeled mGluR3 and GFP-labeled CLC.
  • Movie S5 (.mp4 format). TIRFM of various GPCR molecules on a HEK 293 cell with and without agonist.