Supplementary Materials

This PDF file includes:

  • Fig. S1. Generation of igfbp5a mutant fish.
  • Fig. S2. igfbp5a mRNA levels in mutant fish.
  • Fig. S3. Deletion of Igfbp5a does not alter igfbp5b expression.
  • Fig. S4. igfbp5a Δ4 mutant fish are prone to dying under low Ca2+ stress.
  • Fig. S5. CRISPR-Cas9–mediated transient deletion of Igfbp5a impairs adaptive NaR cell proliferation.
  • Fig. S6. Expression of IGF and insulin ligand and receptor genes in NaR cells.
  • Fig. S7. Deletion of Igfbp5a has no effect on phosphorylated Erk signaling.
  • Table S1. Primers for cloning and sequencing of Igfbp5a, IGFBP5, and their mutants.

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