Supplementary Materials

This PDF file includes:

  • Fig. S1. Validation of the MK2 and 5-LOX antibodies with siRNAs; MerTK deletion does not alter CaMKII activity in the absence of Gas6; and protein S can suppress the CaMKII pathway.
  • Fig. S2. Gas6 reduces cytosolic Ca2+ in a MerTK-dependent manner, and BAPTA-AM suppresses CaMKII–p38–MK2–5-LOX signaling.
  • Fig. S3. Further studies related to MerTK ligands and demonstration that Axl does not mediate Gas6-induced ATP2A2 expression.
  • Fig. S4. Protein S and apoptotic cells activate ERK1/2 in macrophages, and ERK1/2 is required for Gas6-mediated suppression of CaMKII–p38–MK2–5-LOX signaling.
  • Fig. S5. Detection of the cell surface expression of CDMer proteins in transfected HEK 293 cells.
  • Fig. S6. Tyr872 in the cytoplasmic tail of human MerTK is required for the activation of ERK1/2 and CaMKII.
  • Fig. S7. Summary scheme of MerTK-mediated resolution signaling.

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