Supplementary Materials

The PDF file includes:

  • Fig. S1. Construct design for crystallization.
  • Fig. S2. C/EBPα degron electron density.
  • Fig. S3. Dissociation constant of TRIB1/2-binding C/EBPα.
  • Fig. S4. Differing C/EBPα-binding potential between TRIB1 and TRIB3.
  • Fig. S5. Stable regions of C/EBPα degron in complex with TRIB1.
  • Fig. S6. Displacement of the TRIB1 C-terminal tail from COP1.
  • Fig. S7. Destabilization of TRIB1 by Y134C mutation.
  • Fig. S8. PKI screening of TRIB1.
  • Legend for table S1
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). DSF data for individual PKIS compounds.
  • Movie S1 (.mp4 format). Morph between substrate-free (SLE-out) and C/EBPα-bound (SLE-in) structures of TRIB1.
  • Movie S2 (.mp4 format). Simulations of the RIB1–C-terminal tail.