Supplementary Materials

The PDF file includes:

  • Fig. S1. Genes are differentially regulated by egl-9, hif-1, hypoxia, and hir-1.
  • Fig. S2. Expression pattern of hir-1.
  • Fig. S3. Hypoxia- and ECM-related genes similarly impair cuticle integrity.
  • Fig. S4. Role of NHR-49 in hir-1 signaling.
  • Fig. S5. Hypoxia activates comt-5 through a regulatory cascade involving ECM components (LET-756 and DPY-3) and HIR-1.
  • Fig. S6. RNA-seq and qRT-PCR analysis of HIR-1–regulated genes.
  • Fig. S7. Impaired cuticle integrity in hir-1 mutants under various conditions.
  • Table S1. Genes that regulate comt-5p::GFP expression.
  • Table S2. Primer sequences.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). RNA-seq analysis of egl-9, hif-1, and hypoxia-regulated genes.
  • Data file S2 (Microsoft Excel format). Gene ontology and KEGG pathway analyses of genes differentially regulated in hir-1 mutants compared with WT animals.