Supplementary Materials

This PDF file includes:

  • Fig. S1. Similarity of membrane-targeting domains from RRSP and other bacterial toxins.
  • Fig. S2. Dali server search results using RRSP_chainA as the query.
  • Fig. S3. PMT does not cleave rKRAS.
  • Fig. S4. Space-filling model showing cleft with putative active site residues.
  • Fig. S5. EC50 for processing of KRAS + Mg2+.
  • Fig. S6. Divalent cations do not stimulate the activity of chelated rRRSP.
  • Fig. S7. rKRAS* shows localized structural perturbation.
  • Fig. S8. DSF analysis of rRRSP mutants.
  • Fig. S9. SPR of KRAS binding to RRSP.
  • Fig. S10. NMR of KRAS in the presence of rRRSP H4030A.
  • Fig. S11. Schematic representation of the LFNA+PA system.
  • Fig. S12. rtxA1 genes with rrsp genetic modifications induce cell rounding but not RRSP processing in HeLa cells.
  • Table S1. Oligonucleotides used in the study.
  • Table S2. Sequence of gBlocks used in the study.
  • References (7480)

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