Supplementary Materials

The PDF file includes:

  • Fig. S1. Endogenous expression of transcripts encoding GPCRs in HEK293 cells.
  • Fig. S2. Biphasic changes in cAMP are due to activation of endogenous β2AR and M3R.
  • Fig. S3. FRET biosensors can detect responses of endogenous or exogenous receptors to femtomolar concentrations of ligand.
  • Fig. S4. Plasma membrane localization and activity of mutant β2AR and M3R.
  • Fig. S5. Modeling responses to femtomolar concentrations of Iso.
  • Fig. S6. Identification of proteins involved in mediating responses to 1 fM Iso.
  • Fig. S7. The β2AR forms a preassembled signaling complex.
  • Fig. S8. Identification of proteins involved in mediating responses to 1 fM CCh.
  • Fig. S9. The M3R forms a preassembled signaling complex.
  • Fig. S10. Femtomolar ligand concentrations activate compartmentalized signaling and unique cell responses.
  • Legends for tables S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). Femtomolar concentrations of ligand increase cAMP.
  • Table S2 (Microsoft Excel format). The β2AR and M3R constitutively form complexes at the plasma membrane.
  • Table S3 (Microsoft Excel format). Proteins from hierarchical clustering analysis.