Supplementary Materials

This PDF file includes:

  • Fig. S1. Distribution and alteration of TRESK channels in ipsilateral L4/5 DRG neurons of bone lesion–bearing rats.
  • Fig. S2. TRESK antibody validation in HEK293 cells.
  • Fig. S3. Reduction of TRESK-containing currents in IB4 DRG neurons of bone lesion–bearing rats.
  • Fig. S4. Augmented TRESK staining in DRG neurons upon intrathecal administration of lentiviral TRESK.
  • Fig. S5. Effects of TRESK overexpression on the tumor-induced TRESK repression and neuronal hyperexcitability in IB4 DRG neurons from bone lesion–bearing rats.
  • Fig. S6. Effects of intrathecal injection of TRESK siRNA on the expression of other K2P family members in bilateral L4/5 DRGs from normal rats.
  • Fig. S7. Effects of TRESK knockdown on neuronal excitability in IB4 DRGs from normal rats.
  • Fig. S8. Effects of calcineurin inhibition on the abundance of phosphorylated TRESK in DRG neurons.
  • Fig. S9. Effects of interfering peptide TAT-Ser on calcineurin-induced alterations of functional TRESK abundance and neuronal excitability in cultured DRG neurons.
  • Fig. S10. Effects of FK-506 treatment on functional TRESK abundance and neuronal excitability in cultured DRGs and on pain sensitivity in normal rats.
  • Fig. S11. The subcellular distribution of NFAT in cultured DRG neurons upon treatment with FK-506.
  • Fig. S12. Effects of TRESK knockdown on calcineurin-modulated DRG neuron excitability and pain sensitivity in bone lesion–bearing rats.
  • Fig. S13. Effects of intrathecal injection of calcineurin siRNA on functional TRESK abundance, DRG neuron excitability, and pain sensitivity in normal rats.
  • Fig. S14. Effects of intrathecal injection of NFAT inhibitor peptide VIVIT on functional TRESK abundance, DRG neuron excitability, and pain sensitivity in normal rats.
  • Fig. S15. Effects of VEGF treatment on calcineurin and TRESK abundance, TRESK-mediated currents, and the neuronal excitability in cultured DRG neurons.
  • Fig. S16. Effects of VEGF antibody on calcineurin and TRESK abundance, DRG neuron excitability, and pain hypersensitivity in bone lesion–bearing rats.
  • Table S1. Antibodies.
  • Table S2. PCR primer sequences.
  • Table S3. siRNA nucleotide sequences.

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