Supplementary Materials

This PDF file includes:

  • Fig. S1. Response of cells transfected with EPAC reporter and vector to chemokines and RANTES analogs.
  • Fig. S2. Dose-response curves for CCR5-mediated Gi/o protein activation by chemokines and RANTES analogs using cells expressing higher amounts of CCR5.
  • Fig. S3. The effect of chemokines and RANTES analogs on cAMP accumulation in the presence of PTX.
  • Fig. S4. Time courses of CCR5-mediated inhibition of cAMP accumulation induced by chemokines and RANTES analogs.
  • Fig. S5. Response of vector-transfected cells to chemokines and RANTES analogs through stimulation of Ca2+ flux.
  • Fig. S6. The effect of G protein subunit cotransfection on the amount of CCR5 molecules on the cell surface.
  • Fig. S7. ATP-induced Ca2+ flux in HEK293T cells expressing CCR5 and G protein subunits.
  • Fig. S8. The effect of G protein subunit cotransfection on CCR5-mediated Ca2+ flux and IP1 accumulation in response to chemokines and RANTES analogs.
  • Fig. S9. IP1 accumulation in vector-transfected cells in response to chemokines and RANTES analogs.
  • Fig. S10. Dose-response curves for CCR5-mediated Ca2+ flux in response to chemokines and RANTES analogs using cells expressing higher amounts of CCR5.

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