Supplementary Materials

The PDF file includes:

• Fig. S1. Characterization of Rsk2^KO mice.
• Fig. S2. Protein abundance of myosin, RhoGEFs, Gα_q/11/12, MYPT1, MLCK, and ROCK in WT and Rsk2^KO smooth muscle.
• Fig. S3. Demonstration of specificity of Ser²²⁷ and Thr⁵⁷⁷ phospho-specific RSK2 antibodies.
• Fig. S4. RSK2 immunoprecipitated with actin, but not myosin, from mouse abdominal aorta.
• Fig. S5. Typical Ca²⁺ measurements in Fluo-4–loaded cultured normal smooth muscle cells before and after treatment with Na acetate.
• Table S1. Histological analysis of WT and Rsk2^KO mesenteric arteries.
• Table S2. Cardiac functions measured by MRI in WT and Rsk2^KO littermates.
• Legend for Movie S1

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.mp4 format). Ca²⁺ transients in mesenteric arterial smooth muscle.