Supplementary Materials

The PDF file includes:

  • Fig. S1. Characterization of Rsk2KO mice.
  • Fig. S2. Protein abundance of myosin, RhoGEFs, Gαq/11/12, MYPT1, MLCK, and ROCK in WT and Rsk2KO smooth muscle.
  • Fig. S3. Demonstration of specificity of Ser227 and Thr577 phospho-specific RSK2 antibodies.
  • Fig. S4. RSK2 immunoprecipitated with actin, but not myosin, from mouse abdominal aorta.
  • Fig. S5. Typical Ca2+ measurements in Fluo-4–loaded cultured normal smooth muscle cells before and after treatment with Na acetate.
  • Table S1. Histological analysis of WT and Rsk2KO mesenteric arteries.
  • Table S2. Cardiac functions measured by MRI in WT and Rsk2KO littermates.
  • Legend for Movie S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Ca2+ transients in mesenteric arterial smooth muscle.