Supplementary Materials

The PDF file includes:

  • Fig. S1. PP1 and SPAK, but not CaN, act through NBCe1-B Ser65.
  • Fig. S2. CaMKII activates NBCe1-B when expressed in HEK cells.
  • Fig. S3. NBCe1-B phosphorylation sites and the sites affected by IRBIT.
  • Fig. S4. Structural model of NBCe1-B.
  • Fig. S5. Sequence alignment of the NBC family.
  • Fig. S6. Effect of S232D, S233D, and S235D mutations on NBCe1-B(Δ1-95) expression and activity.
  • Fig. S7. Effect of S232X mutation on NBCe1-B expression and activity.
  • Fig. S8. Effect of the S236D mutation in NBCe2-C on transport activity.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). MS data of HEK cells phosphoproteins in the absence of IRBIT.
  • Data file S2 (Microsoft Excel format). MS data of HEK cells phosphoproteins in the presence of IRBIT.</p