Supplementary Materials

This PDF file includes:

  • Text S1. The relationship between the sector, conservation, and allosteric hotspots.
  • Text S2. Background on Kss1.
  • Fig. S1. The effect of surface Asp/Glu-to-Ala mutations on Kss1 expression.
  • Fig. S2. Experimental approach to introduce a PKA phosphorylation site that controls MAPK Kss1 activity.
  • Fig. S3. The kinase sector encompasses the catalytic and regulatory spines.
  • Fig. S4. ERK2 mutations within the kinase sector are enriched for loss of function.
  • Fig. S5. The relationship of negatively charged surface positions to the kinome-wide eukaryotic protein kinase sector.
  • Fig. S6. Phosphorylation, phenotype, and nuclear localization of Hog1-pka mutants.
  • Table S1. Plasmids.
  • Table S2. Yeast strains.
  • Table S3. Comparison of Kss1 point mutations from the literature with our data and the CMGC sector.
  • Table S4. Sector positions mapped to several representative kinase structures.
  • Table S5. Statistical association between the sector, conservation, and ERK2 mutational data.
  • Table S6. Statistical association between the sector, conservation, and KSS1 D/E surface mutations.
  • Table S7. Functional kinase mutations sampled across the kinome.
  • Table S8. Statistical association between the sector, conservation, and functional mutations sampled across a diversity of kinases.
  • References (4259)

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