Supplementary Materials

This PDF file includes:

  • Fig. S1. sAC expression in human melanocytes.
  • Fig. S2. Establishment of Adcy10−/− (sACKO) mouse melanocytes.
  • Fig. S3. Measurement of melanosome pH using DAMP.
  • Fig. S4. sAC regulates organelle pH in melanocytes.
  • Fig. S5. Modulation of sAC-dependent cAMP signaling does not affect melanosome marker fluorescence.
  • Fig. S6. Effects of sAC inhibition on melanosome pH and tyrosinase abundance in human melanocytes.
  • Fig. S7. Effects of distinct sources of cAMP on melanosome pH.
  • Fig. S8. Inhibition of protein synthesis does not affect melanosome pH.
  • Fig. S9. Regulation of melanosome pH in human and mouse melanocytes by distinct cAMP effector proteins.
  • Fig. S10. Pharmacologic inhibition or genetic ablation of sAC increases melanization.
  • Fig. S11. Inhibition of sAC signaling increases eumelanin production.
  • Table S1. Mann-Whitney analysis of median cellular DAMP fluorescence.
  • Table S2. Assessment of Tyr::CRE-ERT2;sACf/f mice.

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