Supplementary Materials

This PDF file includes:

  • Fig. S1. Alignment of mouse FZD5 with the SMO and β2AR sequences corresponding to the crystal structures used for modeling.
  • Fig. S2. Validation of V5-FZD5-FlAsH439-CFP.
  • Fig. S3. Individual traces from single-cell data and kinetic experiments using V5-FZD5-FlAsH436-CFP.
  • Fig. S4. Validation of Gαq and Gαi1 FRET probes with FZD5.
  • Fig. S5. The FZD5 FRET sensors activate Gαq but do not recruit DVL.
  • Fig. S6. Control experiments for DMR in HEK293 cells.
  • Fig. S7. WNT-5A does not activate the WNT–β-catenin pathway in HPAF-II cells.
  • Fig. S8. Pharmacological properties of receptor activation and stimulation of downstream signaling.

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