Supplementary Materials

The PDF file includes:

  • Fig. S1. CCh-evoked Ca2+ signaling in the endothelium.
  • Fig. S2. Flow-induced endothelial Ca2+ signaling.
  • Fig. S3. Ca2+ dependence of CCh- and ATP-evoked endothelial relaxation.
  • Fig. S4. Concentration dependence of the CCh-evoked endothelial Ca2+ response.
  • Fig. S5. Concentration dependence of the ATP-evoked endothelial Ca2+ response.
  • Fig. S6. The sequence of agonist addition does not alter the pattern of cell activation.
  • Fig. S7. Specificity of the antibody recognizing the P2Y2 receptor.
  • Fig. S8. Heterogeneity in NO production.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Regional Ca2+ signals evoked by 100 nM CCh.
  • Movie S2 (.avi format). Spatially distinct cell clusters activated by the EC25 concentrations of CCh and ATP.