Supplementary Materials

The PDF file includes:

  • Fig. S1. Generation of dartboard projection.
  • Fig. S2. Resolution of the STED microscope.
  • Fig. S3. Example of an Orai1−/− T cell with Ca2+ microdomains occurring more deeply within the cytosol.
  • Fig. S4. Markedly decreased initial Ca2+ microdomains in Stim1−/−, Stim2−/−, and Stim1−/−/Stim2−/− T cells.
  • Fig. S5. Comparison of initial Ca2+ microdomains in primary murine T cells in a dartboard projection.
  • Fig. S6. ORAI2 is not involved in the formation of initial Ca2+ microdomains.
  • Fig. S7. Statistical analysis of the formation of initial Ca2+ microdomains in Ryr1−/− and BZ194-treated T cells.
  • Fig. S8. Model for the formation of initial Ca2+ microdomains in T cells.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Initial Ca2+ microdomains in a WT T cell after stimulation.
  • Movie S2 (.mov format). Ca2+ responses in an Orai1−/− T cell upon bead contact.
  • Movie S3 (.mov format). Ca2+ responses in a Stim1−/−/Stim2−/− T cell upon bead contact.