Supplementary Materials

The PDF file includes:

  • Fig. S1. FcεRI-negative RBL-2H3 cells move like WT cells, and PLL leads to FcεRIγ phosphorylation in RBL-2H3 cells and primary basophils.
  • Fig. S2. Equivalent FcεRI-dependent binding is exhibited by all Fcε-containing ligands used for RBL-2H3 cell activation, and FcεRIγ phosphorylation in primary basophils is regulated by the global kinase-phosphatase balance.
  • Fig. S3. Additional examples of CD45 exclusion from the basal planes of RBL-2H3 cells on ligand-presenting surfaces.
  • Fig. S4. Syk is recruited to FcεRI bound to surface-associated ligand, and robust CD45 exclusion in primary basophils requires specific IgE-ligand interactions.
  • Fig. S5. CD45 exclusion is a passive process.
  • Fig. S6. Integrin activity is not required for CD45 exclusion or FcεRI triggering in either RBL-2H3 cells or primary basophils.
  • Fig. S7. Reducing the size of CD45 or increasing the size of ligand impairs both CD45 exclusion and FcεRI triggering.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). CD45 is excluded from regions of FcεRI-ligand interactions on glass.
  • Movie S2 (.mov format). CD45 exclusion is not affected by depletion of the β integrin CD29.
  • Movie S3 (.avi format). Decreasing the extracellular domain size of CD45 impairs its exclusion from regions of FcεRI-ligand interactions.