Supplementary Materials

This PDF file includes:

  • Fig. S1. Reduced respiratory capacity but increased glycolytic activity in iNKT cells than in CD4 T cells.
  • Fig. S2. Metabolic reprogramming after long-term activation.
  • Fig. S3. 2-DG inhibits IFN-γ production and TCR recycling in iNKT cells activated by anti-CD3 plus anti-CD28.
  • Fig. S4. 2-DG does not reduce cell numbers of iNKT cells.
  • Fig. S5. Influences of 2-DG on proliferation and activation markers of iNKT cells activated by PMA plus ionomycin.
  • Fig. S6. Shortening TCR signaling inhibits IFN-γ production.
  • Fig. S7. 2-DG inhibits TCR signaling proteins even after TCR engagement.
  • Fig. S8. 2-DG inhibits gene transcription.
  • Fig. S9. 2-DG does not influence accumulation of TCR at synapses of CD4 T cells.
  • Fig. S10. Inhibitors do not influence the expression of HK-II.
  • Fig. S11. Inhibition of glycolysis reduces translocation of mTOR to lysosomes.
  • Fig. S12. PEP and d-mannose do not restore IFN-γ production in 2-DG–treated iNKT cells.
  • Table S1. List of primer sequences.

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