Supplementary Materials

This PDF file includes:

  • Fig. S1. Exemplary gating strategy used in flow cytometry analysis to detect and quantify apoptotic and necrotic cells.
  • Fig. S2. HCmel12 cells display intrinsic Gq activity.
  • Fig. S3. Mouse B16 melanoma cells exhibit increased levels of Gq activity.
  • Fig. S4. FR does not affect total ERK protein abundance in HCmel12, B16, or HEK293 cells.
  • Fig. S5. Functional expression of WT and mutant Gαq proteins in an SRE reporter assay.
  • Fig. S6. Authentication of UM cell lines by Sanger sequencing at position Q209 of Gαq.
  • Fig. S7. Effects of AlF4 on IP1 production in UM cell lines expressing WT or mutant Gαq.
  • Fig. S8. FR inhibits AlF4-mediated IP1 accumulation in Mel290 cells.
  • Fig. S9. FR and trametinib do not alter the abundance of total ERK1/2 proteins in UM cell lines.
  • Fig. S10. Time course analysis of the amounts of total AKT and pAKT in UM cell lines after treatment with FR.
  • Fig. S11. Neither FR nor forskolin alters the abundance of total cellular YAP protein.

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