Supplementary Materials

This PDF file includes:

• Fig. S1. Current/voltage (I/V) curve comparison of full-length and truncated CsR-WT in X. laevis oocytes.
• Fig. S2. Overall crystal packing of CsR-WT.
• Fig. S3. Quality of the electron density of the chromophore retinal, Arg⁸³, and Tyr¹⁴ in CsR-WT.
• Fig. S4. Structural superposition of CsR-WT and BR.
• Fig. S5. Close-up views of the structural arrangement around the conserved Arg in various microbial rhodopsins.
• Fig. S6. pH titration of Asp⁸⁶ in CsR-R83Q.
• Fig. S7. Single wavelength traces of photochemical Y14F and Y14E.
• Fig. S8. Current/voltage (I/V) curves of stationary photocurrents at different extracellular salt conditions.
• Fig. S9. Steady-state FTIR measurements of CsR-WT and CySeR.
• Fig. S10. Calculated absolute spectra of the photocycle intermediates of CsR-WT.
• Table S1. Time constants of photocycle transitions in CsR-WT and CySeR.
• Reference (75)

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