Supplementary Materials

This PDF file includes:

  • Fig. S1. Generation of Hspa4l−/− mice.
  • Fig. S2. Hspa4l−/− male mice of a mixed strain are not sterile.
  • Fig. S3. Histological analysis of testes.
  • Fig. S4. The numbers of spermatogonia and sperm are normal in Hspa4l−/− mice.
  • Fig. S5. Subcellular localization of Ppp1cc2.
  • Fig. S6. Ppp1cc2 forms a chaperone-substrate complex with Hsp70.
  • Fig. S7. Subcellular analysis of the interaction of Ppp1cc2 with Hsc70.
  • Fig. S8. Generation of Prm2S56A/wt and Prm2S56D/wt mice and demonstration that Prm2S56A/S56A and Prm2S56D/S56D male mice are fertile.
  • Fig. S9. No mutations in potential off-target sites.
  • Fig. S10. No mutations in neighboring sites of mutated regions.
  • Fig. S11. Morphological analysis of sperm.
  • Table S1. Sperm binding assays.
  • Table S2. Development of oocytes in culture after fertilization with sperm from Hspa4l−/− mice.
  • Table S3. Development of oocytes in culture after intracytoplasmic injection of sperm.
  • Table S4. Development of oocytes in culture after injection with round spermatids.
  • Table S5. Development of oocytes in culture after fertilization with sperm from Prm2S56A/S56A mice.
  • Table S6. Development of oocytes in culture after fertilization with sperm from Prm2S56D/S56D mice.
  • Table S7. Development of oocytes in culture after fertilization with sperm from Hspa4l−/−; Prm2S56A/S56A mice.
  • Table S8. Development of oocytes in culture after fertilization with sperm from Hspa4l−/−; Prm2S56D/S56D mice.
  • Table S9. Primers used for sequencing potential off-target sites.

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