Supplementary Materials

The PDF file includes:

• Fig. S1. Adhesin expression in S. gordonii cells isolated from saliva and plaque.
• Fig. S2. Growth curves of S. gordonii strains.
• Fig. S3. Biofilm biomass of PavB mutant strains.
• Fig. S4. Additional examples of colocalization of LPXTG motif C-peps with the cell membrane.
• Fig. S5. pavB, SGO_1181, and SGO_1180 are cotranscribed.
• Fig. S6. ScaA contributes to enhanced biofilm formation of ∆SspAB S. gordonii.
• Fig. S7. Conservation of SGO_1180 homologs across streptococci.
• Fig. S8. Conservation of SGO_1180 homologs in Firmicutes.
• Table S1. Characteristics of potential S. gordonii sensor HKs.
• Table S2. Transcriptome and RT-qPCR fold change in gene expression values for biofilms.
• Table S3. RT-qPCR fold-change gene expression values for free-growing bacteria.
• Table S4. Oligonucleotides used in this study.
• Table S5. Bacterial strains used in this study.
• Table S6. Plasmids used in this study.
• Legends for movies S1 and S2
• Reference (67)

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.avi format). Colocalization of C-pep in the S. gordonii cell membrane.
• Movie S2 (.avi format). Colocalization of C-pep in the S. gordonii cell membrane at high magnification.