Supplementary Materials

The PDF file includes:

  • Fig. S1. PC2 KD and PC2 KO result in increased mitochondrial Ca2+ signals.
  • Fig. S2. PC2 resides in ER membranes close to mitochondria.
  • Fig. S3. PC2 KD does not alter the expression of MCU or multiple MCU complex components.
  • Fig. S4. MFN2 KD rescues Ca2+ signaling in PC2 KD cells and PC2 KD alters mitochondrial metabolism and density.
  • Fig. S5. Super-resolution STED microscopy does not alter mitochondrial ROS production.
  • Fig. S6. PC2 KD does not alter the expression of alternative ER-mitochondrial linkers.
  • Fig. S7. LV-siMFN2 is effective at knocking down MFN2 in cells from Pkd2F/FPkhd1Cre mice.
  • Fig. S8. LV-siMFN2 treatment rescues multiple effects of PC2 deficiency in cystic cells.
  • Fig. S9. Mitochondrial density is increased in cyst-lining cells from ADPKD kidneys.
  • Legend for movie S1

[Download PDF]

Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Super-resolution movie of organelle dynamics.