Supplementary Materials

This PDF file includes:

  • Fig. S1. Alignment of GarA homologs in selected Actinobacteria.
  • Fig. S2. Continuous sedimentation coefficient distribution analysis of the PknBCD-GarA complex.
  • Fig. S3. ITC characterization of the interaction between autophosphorylated PknBCD and GarA.
  • Fig. S4. The N-terminal GarA extension occupies a similar position in different GarA structures.
  • Fig. S5. Conserved mode of phosphopeptide recognition in different M. tuberculosis FHA domains.
  • Fig. S6. Detailed structure of the PknB activation loop bound to GarA.
  • Fig. S7. Formation of the enamine-ThDP covalent adduct in the presence of GarA.
  • Fig. S8. SPR studies of protein-protein interactions.
  • Table S1. Data collection and refinement statistics.
  • References (64, 65)

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