Supplementary Materials

This PDF file includes:

  • Fig. S1. Lack of effect of mTOR inhibition on α-SMA induction and the serine-glycine biosynthetic pathway, respectively.
  • Fig. S2. Knockdown and knockout controls and evidence that TGF-β1 stimulation in fibroblasts is not associated with PERK activation.
  • Fig. S3. ATF4 knockdown abrogates the TGF-β1–induced increase in glycine biosynthesis enzymes.
  • Fig. S4. TGF-β1 stimulation increases glucose uptake and abundance of SLC2A1 transcripts, GLUT1, and glycolytic enzymes.
  • Fig. S5. Exogenous serine does not rescue the inhibitory effects of ATP-competitive mTOR inhibition on TGF-β1–induced collagen deposition.
  • Table S1. MetaCore pathways enriched in the rapamycin-insensitive mTOR module.
  • Table S2. Primer sequences.

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