Supplementary Materials

The PDF file includes:

  • Fig. S1. Astrocytes exhibit SOCE mediated by Orai1 and STIM1.
  • Fig. S2. Stimulation of purinergic and PAR GPCRs activates SOCE in hippocampal astrocytes.
  • Fig. S3. Kinetics of individual spH fusion events in WT astrocytes.
  • Fig. S4. spH-monitored vesicular exocytosis in AWESAM stellate astrocyte cultures.
  • Fig. S5. Local Ca2+ signals contribute to CRAC channel–mediated vesicular exocytosis.
  • Fig. S6. Exocytosis as monitored by FM1-43 is impaired in Orai1 KO astrocytes.
  • Fig. S7. Ca2+ fluctuations in astrocyte processes in situ.
  • Legends for movies S1 to S4
  • References (75, 76)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). spH-monitored exocytotic events in a WT astrocyte.
  • Movie S2 (.avi format). spH-monitored exocytotic events in an Orai1 KO (Orai1fl/fl GFAP-Cre) astrocyte.
  • Movie S3 (.avi format). GCaMP6f signals monitored by 2PLSM in an astrocyte from a WT hippocampal slice.
  • Movie S4 (.avi format). GCaMP6f signals in an astrocyte from an Orai1 KO (Orai1fl/fl GFAP-Cre) hippocampal slice.