Supplementary Materials

The PDF file includes:

  • Fig. S1. Translocation of phospho-MLKL onto lipid rafts upon TSZ treatment of HT-29 cells.
  • Fig. S2. The N-terminal helix bundle of MLKL was essential for its association with flotillins and for subsequent flotillin-mediated endocytosis.
  • Fig. S3. Four SNPs in the human FLOT1 gene compromised the association between flotillin-1 and MLKL and sensitized cells to TSZ-induced necroptosis.
  • Fig. S4. Knockout of either ALIX or SDCBP in HT-29 cells enhanced necroptotic cell death.
  • Fig. S5. Concomitant knockout of SDCBP and FLOT2 did not affect the TNFα signaling pathway.
  • Fig. S6. MLKL associated with TSG101, ALIX, and syntenin-1 through its N-terminal helix bundle.
  • Fig. S7. Knockout of Flot2 but not of Alix or Sdcbp sensitized L929 cells to TZ-induced necroptosis.
  • Legend for movie S1
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). SDCBP and FLOT2 double-knockout cells were more sensitive to TSZ-induced necroptosis than wild-type HT-29 cells.
  • Data file S1 (Microsoft Excel format). Mass spectrometry dataset for the phospho-MLKL affinity purification from lipid rafts in DSP–cross-linked cells.