Supplementary Materials

This PDF file includes:

  • Fig. S1. AMPK associates with and phosphorylates mTOR within mTORC2.
  • Fig. S2. AMPK promotes mTORC2 signaling in response to energetic stress induced by diverse agents in cultured cells.
  • Fig. S3. Time course experiments examining the effects of glucose withdrawal, phenformin, and rotenone on AMPK activation, mTORC2 signaling, and mTOR phosphorylation.
  • Fig. S4. AMPK promotes mTORC2 signaling in liver in vivo and skeletal muscle ex vivo.
  • Fig. S5. TOPO cloning, sequencing, and genotyping of mTORA/A mice bearing mTOR Ser1261 knock-in alleles using CRISPR-Cas9–mediated genome editing.
  • Fig. S6. AMPK and Akt promote cell survival during acute energetic stress.

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