Supplementary Materials

This PDF file includes:

  • Fig. S1. Knockdown efficiency of shRNAs and off-target tests in palatal shelves organ culture.
  • Fig. S2. Knockdown of HIPK2 decreases palate fusion at 48 hours after infection.
  • Fig. S3. Loss of kinase activity of the HIPK2-K221R protein.
  • Fig. S4. Knockdown of p21 inhibits HIPK2-promoted apoptosis and palate fusion at 48 hours after infection.
  • Fig. S5. Ectopic expression of HIPK2 does not affect the expression of EMT markers during palate fusion.
  • Fig. S6. Ectopic expression of HIPK2 represses p63 accumulation only in MEE cells during palate fusion.
  • Fig. S7. TAK1 and phosphorylated TAK1 in palatal shelves during development.
  • Table S1. Primers for RT-PCR.
  • Table S2. Primers for ChIP-PCR.
  • Table S3. Antibodies and dilutions.

[Download PDF]