Supplementary Materials

The PDF file includes:

  • Fig. S1. Characterization of other candidates showing increased abundance after 2DG treatment.
  • Fig. S2. Hog1 signaling responds to 2DG, but DOG1 expression is not regulated by Hog1.
  • Fig. S3. DOG1 expression is regulated by the UPR pathway.
  • Fig. S4. Slt2 participates in the regulation of DOG1 expression.
  • Fig. S5. Cis regions involved in the regulation of the DOG2 promoter by glucose.
  • Fig. S6. Identification of reg1 and hxk2 mutants within the isolated spontaneous 2DG-resistant mutants.
  • Fig. S7. Multiple protein sequence alignment of Hxk2 orthologs and positions of the mutations identified.
  • Fig. S8. HDHD1 but not its close homologs HDHD4 or PSPH allow resistance to 2DG.
  • Table S1. Single-nucleotide variants in clones #9 and #10 as compared to the WT strain, as identified by whole-genome resequencing.
  • Table S2. Yeast strains used in this study.
  • Table S3. Plasmids used in this study.
  • Table S4. Antibodies used in this study.
  • References (117121)

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Proteomic response to 2DG treatment.