Supplementary Materials

This PDF file includes:

  • Fig. S1. DGKζ KO T cells display impaired TH2 differentiation in vitro.
  • Fig. S2. DGKζ KO T cells display attenuated TH2 differentiation at early time points.
  • Fig. S3. Addition of exogenous IL-4 restores TH2 differentiation in DGKζ KO T cells.
  • Fig. S4. DGKα KO mice are partially protected from OVA-induced allergic airway inflammation and AHR.
  • Fig. S5. Loss of DGKζ selectively impairs the ability of T cells to produce IL-4 in a T cell–intrinsic manner during TH2 differentiation in vivo.
  • Fig. S6. Myh11-Cre is not a driver of the reduction in tension of Myh11-Cre DGKfl/fl tracheal rings in response to methacholine.
  • Fig. S7. Inhibition of ERK signaling is sufficient to restore TH2 differentiation in DGKζ KO T cells.
  • Table S1. Flow cytometry antibodies.
  • Table S2. Immunoblotting antibodies.

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