Supplementary Materials

This PDF file includes:

  • Fig. S1. β-Arrestin recruitment to ACKR3WT induced by CCX662 and CXCL11 in BRET experiments.
  • Fig. S2. Expression of ACKR3 variants in HEK cells.
  • Fig. S3. Kinetics of association of CXCL12 with ACKR3 on live Sf9 cells.
  • Fig. S4. SPR in nanodiscs and detergent micelles.
  • Fig. S5. N-terminal cytochrome b562-RIL fusion protein did not affect CXCL12 dissociation rate or folding of ACKR3.
  • Fig. S6. BRET signal increase after addition of 2 μM chemokine for ACKR3WT and ACKR3d29.
  • Table S1. IC50 values (nM) for equilibrium binding studies reported in literature.
  • References (4751)

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