Supplementary Materials

This PDF file includes:

  • Fig. S1. Map of signaling networks analyzed in distinct cell populations.
  • Fig. S2. Response of αβ T cells to stimulus.
  • Fig. S3. Network behavior of activated αβ T or CD19-28 ζ CAR-T cells.
  • Fig. S4. Representative DREVI plots for expanded CD8+ T cells.
  • Fig. S5. DREMI scores for expanded transduced and nontransduced T cells.
  • Fig. S6. Representative DREVI plots for expanded transduced and nontransduced T cells.
  • Fig. S7. TIM-3 abundance in expanded transduced and nontransduced αβ T cells.
  • Fig. S8. Phosphoprotein abundance in CD28-CCRs in Vδ2+ γδT cells.
  • Fig. S9. TNFα production in response to CCR stimulus.
  • Fig. S10. DREMI scores in expanded or CD33-DAP10 CCR+ γδT cells.
  • Fig. S11. SYK, ABL, and ZAP70 activity are increased in Vδ2+ γδT cells cultured with AML cells.
  • Fig. S12. CD33-DAP10 CCR+ Vδ2+ γδT cells produce cytokines in response to AML cells.
  • Fig. S13. Schematics of CAR and CCR constructs used in this study.
  • Fig. S14. Cartoon illustrating stimulation of CAR-transduced αβ T cells by antibody cross-linking.
  • Fig. S15. Flow and mass cytometry gating strategies.
  • Table S1. Statistical analysis of data displayed in Fig. 3H (CD4+).
  • Table S2. Statistical analysis of data displayed in Fig. 3H (CD8+).
  • Table S3. Statistical analysis of data displayed in fig. S5.
  • Table S4. Antibodies used in mass cytometry.
  • Table S5. Antibodies used in flow cytometry.

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