Supplementary Materials

This PDF file includes:

  • Fig. S1. PGE2 dose response in monocytes treated with MDP alone or with MDP in the presence of Tc CM.
  • Fig. S2. CM from CD3 bead–treated, negatively selected T cells increased PGE2 production in monocytes activated with MDP.
  • Fig. S3. Tc CM strongly augmented production of IL-8 and only minimally increased that of RANTES and IL-12 p40 in monocytes activated with MDP.
  • Fig. S4. Production of PGE2 in monocytes activated with MDP and Tc CM is IL-1β independent.
  • Fig. S5. CM from CD3 bead–treated, negatively selected T cells increased COX2 mRNA expression in monocytes activated with MDP.
  • Fig. S6. Production of IL-1β and IL-6 in monocytes activated with MDP and Tc CM is sensitive to RIP2 kinase inhibitor erlotinib.
  • Fig. S7. Western blot of NF-κB p65 in nuclear extracts of monocytes activated with MDP and Tc CM in the absence or presence of erlotinib.
  • Fig. S8. Tc CM but not NSTc CM induced calcium flux in monocytes.
  • Fig. S9. Flow cytometry of bead-purified T cells with antibodies to CD41a.
  • Fig. S10. Gating strategy to exclude presence of CD41a+ platelets in subsets of sorted CD4+ and CD8+ T cells.
  • Fig. S11. CM from sort-purified T cells incubated with CD3 beads overnight induced COX2 mRNA and PGE2 in monocytes activated with MDP.
  • Fig. S12. Platelets did not induce PGE2 in MDP-treated monocytes.
  • Fig. S13. Cox2 mRNA Ct values in the spleen and liver of WT, CD18 KO, and αM KO mice injected with MDP or PBS.
  • Fig. S14. rGPIbα increased PGE2, IL-1β, and IL-6 in MDP-treated monocytes.
  • Fig. S15. Western blot of NF-κB p65 in nuclear extracts prepared from monocytes activated with MDP and rGPIbα.
  • Table S1. Production of PGE2 in monocytes activated with MDP and Tc CM but not with MDP and NSTc CM.
  • Table S2. Increased PGE2-inducing activity in concentrated Tc CM.
  • Table S3. List of proteins increased twofold or higher in Tc CM compared with NSTc CM.

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