Supplementary Materials

The PDF file includes:

• Fig. S1. Sequence alignments.
• Fig. S2. Generation and characterization of KCC2 Thr⁹⁰⁶/Thr¹⁰⁰⁷ phosphomimetic mice.
• Fig. S3. Hematoxylin and eosin staining of horizontal and sagittal midline sections of P0 mice showing no gross defects of the central nervous or the musculoskeletal system.
• Table S1. In vivo KCC2 phosphorylation sites identified and quantified in all four conditions (E18.5, P0, P20, and adult).
• Table S2. List of candidate protein kinases.
• Legends for movies S1 to S4
• Reference (75)

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Other Supplementary Material for this manuscript includes the following:

• Movie S1 (.m4v format). Seizure triggered by mild brush stroke in a homozygous P0 Kcc2^E/E mouse.
• Movie S2 (.m4v format). Seizure triggered by tail pinch in a homozygous P0 Kcc2^E/E mouse.
• Movie S3 (.m4v format). Seizure triggered by tail suspension in a homozygous P0 Kcc2^E/E mouse.
• Movie S4 (.m4v format). Spontaneous seizure in a homozygous P0 Kcc2^E/E mouse.