Supplementary Materials

The PDF file includes:

  • Fig. S1. ATOH8 is induced by BMP-9/ALK-1 but not by Notch in ECs.
  • Fig. S2. Zebrafish atoh8 is expressed in large vessels, and atoh8 morphants have slight delay in ISV formation.
  • Fig. S3. Atoh8−/− mice are small in body size but are viable and fertile.
  • Fig. S4. Atoh8 is expressed in ECs in the lung, and Atoh8−/− mice exhibit PAH-like phenotype.
  • Fig. S5. ATOH8 interacts with HIF-1α and decreases its protein abundance.
  • Legends for data files S1 to S4

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Gene regulation of 70 putative direct target genes of BMP-9/ALK-1.
  • Data file S2 (Microsoft Excel format). SMAD1/5-binding sites in HUVECs that are related to blood vessels or the heart.
  • Data file S3 (Microsoft Excel format). Genomic regions commonly bound by FLAG-ATOH8 in HPAEC-FLAG-ATOH8 cells.
  • Data file S4 (Microsoft Excel format). Primer sets used for qRT-PCR and ChIP-qPCR.