Supplementary Materials

This PDF file includes:

  • Fig. S1. DKK1 induces endocytosis of CKAP4.
  • Fig. S2. Palmitoylation of CKAP4 at Cys100 is not essential for ER exit or binding to DKK1.
  • Fig. S3. ZDHHC5 palmitoylates CKAP4 in the cell surface.
  • Fig. S4. Knockdown of APTs or inhibition of PI3K-AKT signaling inhibits DKK1-induced depalmitoylation of CKAP4.
  • Fig. S5. CKAP4 interacts with PI3K in the detergent-resistant fractions of the plasma membrane.
  • Fig. S6. Depalmitoylation of LRP6 does not affect DKK1-dependent inhibition of Wnt–β-catenin signaling.
  • Fig. S7. CKAP4 is not involved in the DKK1-induced inhibition of Wnt–β-catenin signaling.
  • Fig. S8. Wnt–β-catenin signaling is not activated in Capan-1 cells.
  • Fig. S9. DKK1, CKAP4, and LRP5 are highly expressed in human pancreatic cancer tissues.
  • Fig. S10. Schematic model of DKK1-CKAP4 signaling.
  • Table S1. Expression of CKAP4, DKK1, LRP5, and LRP6 in tumor and normal samples from public datasets.
  • Table S2. List of antibodies used in this study.
  • Table S3. List of materials and chemicals used in this study.
  • Table S4. List of plasmids used in this study.
  • Table S5. List of cell lines used in this study.
  • Table S6. List of target sequences for siRNAs.
  • Table S7. List of primer sequences for quantitative PCR.

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