Supplementary Materials

The PDF file includes:

  • Fig. S1. NFAT activation by constitutively active STIM1 mutants.
  • Fig. S2. Constitutive CAD dissociation for the STIM1-H72R mutant.
  • Fig. S3. Ca2+ ion binding to the EF-SAM domain.
  • Fig. S4. Ca2+-binding probability and RMSD values for the STIM1 canonical EF-hand mutants.
  • Fig. S5. Altered protein unfolding for the STIM1-F108I mutant.
  • Legends for movies S1 and S2

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mov format). Multiple Ca2+-binding events to the EF-hand domains of STIM1.
  • Movie S2 (.mov format). A single bound Ca2+ ion maintains the luminal STIM1 structure.