Supplementary Materials

The PDF file includes:

  • Fig. S1. cGAS does not associate with G3BP1 and is not required for SG formation after heat shock.
  • Fig. S2. cGAS colocalizes with G3BP1 in HFF cells.
  • Fig. S3. cGAS Cys396 and Cys397 are required for G3BP1 association.
  • Fig. S4. cGAS KO did not affect arsenite stimulation of SG formation.
  • Fig. S5. G3BP1 binds to the N-terminal region of cGAS.
  • Fig. S6. The cGAS activity is not required for G3BP1 association.
  • Fig. S7. Loss of PKR or G3BP1 prevents DNA-stimulated interferon production.
  • Fig. S8. Loss of PKR or G3BP1 prevents ISD-stimulated Ifnβ transcription in HFF cells.
  • Fig. S9. Knockdown of TIA1 does not affect DNA-induced p-IRF3 expression.
  • Fig. S10. Knockdown of cGAS, PKR, or G3BP1 does not impair induction of interferon production by cGAMP.
  • Fig. S11. Loss of PKR or G3BP1 prevents DNA virus particle–stimulated interferon production.
  • Fig. S12. The sizes of arsenite- and ISD-induced G3BP1-containing foci.
  • Fig. S13. Schematic presentation of the coding regions of cGAS, PKR, G3BP1, and STING that are targeted by CRISPR-Cas9.
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). cGAS-associated cellular proteins through performing co-IP followed by mass spectrometry and proteomic analysis in 293T cells.