Supplementary Materials

This PDF file includes:

  • Fig. S1. Selection of NK cell clones expressing receptors of interest for imaging.
  • Fig. S2. Data processing controls for STORM data.
  • Fig. S3. Inhibitory receptors are clustered according to STORM analysis methods.
  • Fig. S4. The abundances of KIR2DL3 and KIR3DL1 correlate with their nanoscale organization.
  • Fig. S5. Allotypic differences in KIR3DL1 do not affect its nanoscale organization when its abundance is controlled.
  • Fig. S6. The abundance of KIR3DL1 determines its nanoscale organization.
  • Fig. S7. The abundance of KIR2DL1 determines its nanoscale organization in YTS cells, as assessed by STORM.
  • Fig. S8. In silico model of the effects of increasing KIR2DL1 density reveals that the receptors must be added into existing clusters to enlarge the cluster area.
  • Fig. S9. YTS cells expressing different amounts of KIR2DL1 can be equally inhibited.
  • Fig. S10. Inhibition of SHP-1/2 does not change the organization of KIR2DL1 in YTS-KIRlow or KIRhigh cells.
  • Table S1. Primers for the cloning of inhibitory receptors.
  • Table S2. Primers for the genomic investigation of KIR2DL1.
  • Table S3. Primers for differentiating KIR2DL1 alleles expressed in pNK clones.

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