Supplementary Materials

This PDF file includes:

  • Fig. S1. Quantitation of TGF-β–induced HK2 accumulation in murine and human fibroblasts and mitochondrial translocation of HK2.
  • Fig. S2. Quantitation of profibrotic proteins in AKR-2B and TIG-1 cells after HK2 knockdown.
  • Fig. S3. HK2 knockdown does not affect TGF-β–induced activation of Smad3 or Akt.
  • Fig. S4. Effect of Lonidamine on HK activity, production, cell viability, and TGF-β signaling.
  • Fig. S5. Quantitation of profibrotic TGF-β targets in AKR-2B and TIG-1 cells treated with Lonidamine.
  • Fig. S6. TGF-β–induced HK2 production is mediated by EGFR and PDGFR but not the mTOR pathway.
  • Fig. S7. HK inhibition improves lung structure, total collagen content, profibrotic gene expression, and tissue compliance in BLM-induced lung fibrosis.
  • Table S1. Pharmacological inhibitors.
  • Table S2. Commercial antibodies.
  • Table S3. Quantitative PCR murine primers.

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