Supplementary Materials

This PDF file includes:

• Fig. S1. TNFR1 immunoprecipitations with Flag-tagged TNFα reveal the predominant ligand-interacting TNFR1 isoforms.
• Fig. S2. Analysis of TNFα/TNFR1-mEos2 binding in MEFs by flow cytometry and TNFα binding assays.
• Fig. S3. The concept of quantitative SMLM.
• Fig. S4. Functional characterization of SNAP-Flag-TNC-TNFα.
• Table S1. Statistical analysis of qPALM (quantitative PALM) data for unliganded WT TNFR1.
• Table S2. Statistical analysis of qPALM data for stimulated WT TNFR1 that colocalized with TNFα.
• Table S3. Statistical analysis of qPALM data for stimulated WT TNFR1 that did not colocalize with TNFα.
• Table S4. Statistical analysis of qPALM data for unliganded TNFR1 K32A.
• Table S5. Statistical analysis of qPALM data for stimulated TNFR1 K32A.
• Table S6. Statistical analysis of qPALM data for unliganded TNFR1 N66F.
• Table S7. Statistical analysis of qPALM data for stimulated TNFR1 N66F.

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