Supplementary Materials

This PDF file includes:

  • Fig. S1. The ncNF-κB pathway.
  • Fig. S2. CAR expression on T cells from three donors expanded by irradiated Nalm6 cells.
  • Fig. S3. CAR expression on T cells from three donors expanded by anti-CD19 beads.
  • Fig. S4. CAR expression on T cells from three donors activated by anti-CD19 beads for signaling assays.
  • Fig. S5. Anti-CD19 BBζ CAR activation induces T cell signaling.
  • Fig. S6. Additional representative Western blotting analysis of nuclear and cytoplasmic fractions to compare anti-CD19 CAR signaling.
  • Fig. S7. Anti-mesothelin BBζ CAR also drives ncNF-κB signaling.
  • Fig. S8. Dual-transduced T cell production scheme, day 0 CAR, and mCherry expression.
  • Fig. S9. Western blotting analysis of nuclear and cytoplasmic fractions to compare control and dnNIK-expressing anti-CD19 BBζ T cells.
  • Fig. S10. dnNIK does not affect PCNA abundance in CAR T cells.
  • Fig. S11. BimEL abundance in anti-CD19 CAR T cells.
  • Fig. S12. ERK1/2 phosphorylation in control and dnNIK-expressing BBζ CAR T cells.
  • Fig. S13. FOXO3a abundance and phosphorylation in control and dnNIK-expressing BBζ CAR T cells.
  • Fig. S14. CAR constructs.
  • Fig. S15. Representative gating strategy to quantify cell death by flow cytometry and assess the elimination of CD19+ Nalm6 cells.

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