Supplementary Materials

The PDF file includes:

  • Fig. S1. Validation of the Polκ antibody.
  • Fig. S2. MAPK inhibition in melanoma cell lines increases POLK mRNA levels and changes the subcellular localization of Polκ.
  • Fig. S3. DNA damage is not associated with the subcellular shift of Polκ.
  • Fig. S4. Cell cycle inhibition is not responsible for the shift in the subcellular localization of Polκ.
  • Fig. S5. MAPK inhibition decreases the abundance of p-S6.
  • Fig. S6. BRAF inhibition induces ER stress, and other inducers of ER stress also change the subcellular localization of Polκ.
  • Fig. S7. The effects on POLK mRNA levels are driver gene specific across tumor types.
  • Fig. S8. Glucose starvation also modulates the subcellular localization of Polκ.
  • Fig. S9. Inhibiting importin-β does not prevent the nuclear shift of Polκ.
  • Fig. S10. Inhibition of the proteosome does not have a differential effect on the protein level of Polκ after treatment with DMSO or PLX4032.
  • Table S1. qRT-PCR primers used in the study.
  • Table S2. sgRNAs and validation primers used for MMR and Polκ KO.
  • Table S3. RNA-seq and GSEA analysis on Polκ-CRISPR A375 cells.

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Other Supplementary Material for this manuscript includes the following:

  • Data file S1 (Microsoft Excel format). Differentially expressed genes between A375 cells with POLK sgRNA versus control sgRNA.