Supplementary Materials

The PDF file includes:

  • Fig. S1. Flagellar motility promotes colony expansion and engulfment of foreign objects.
  • Fig. S2. Motile cells move forward during engulfment, followed by matrix producers.
  • Fig. S3. Expression of the flagellin GFP reporter in colonies over time.
  • Fig. S4. Formation of TasA-mCherry fibers in the microfluidics system.
  • Fig. S5. Osmotic pressure does not affect growth rate or restore hag expression in the ΔtasA mutant.
  • Fig. S6. Complementation of a tasA mutant by tapA-sipW-tasA expression in trans.
  • Fig. S7. ECM-expressing cells form chains in ΔtasA.
  • Fig. S8. TasA stimulates the reversal to motility by antagonizing tapA expression.
  • Fig. S9. TasA stimulates the reversal to motility independently of DegU or KinD.
  • Fig. S10. The effects of KinA to KinC on TasA-dependent motility expression.
  • Fig. S11. The CssR/CssS two-component system.
  • Fig. S12. Genes differentially expressed in both ΔtasA and ΔcssRS.
  • Fig. S13. Stress response genes regulated by TasA and CssR/CssS.
  • Fig. S14. TasA stimulates the transition from a matrix-producing state back to the motile state.
  • Table S1. Bacterial strains.
  • Table S2. Primers.
  • Legends for movies S1 to S3
  • Legend for data file S1

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). TasA fiber formation.
  • Movie S2 (.mp4 format). Induction of motility occurs after tasA expression.
  • Movie S3 (.mp4 format). TasA stimulates reversal to motility after entering the biofilm state.
  • Data file S1 (Microsoft Excel format). Gene expression analysis.