Supplementary Materials

This PDF file includes:

  • Fig. S1. Time traces of Hog1 nuclear translocation with different break times.
  • Fig. S2. Data processing workflow for quantification of memory effects as a function of the break time.
  • Fig. S3. The effect of priming input on stress adaptation in single cells.
  • Fig. S4. A priming input with 3 μM 1-NM-PP1 cannot induce the memory effect in cells with WT PKA.
  • Fig. S5. Memory dynamics in response to 45-min 0.5 M KCl priming input.
  • Fig. S6. Time traces of Hog1 nuclear translocation with different break times when 0.5 M KCl is used as the priming input.
  • Fig. S7. Biphasic memory effects on the adaptation to glucose limitation.
  • Fig. S8. Time traces of Msn2 nuclear translocation with different break times when glucose limitation is the second stress.
  • Fig. S9. The effect of 15-min 3 μM 1-NM-PP1 on stress adaptation time is statistically significant.
  • Fig. S10. Short-lived memory is regulated by the trehalose-degrading enzyme Nth1.
  • Fig. S11. Identification of the transcription factors Msn2/4 and Yap1 in mediating long-lived memory.
  • Fig. S12. Colocalization of DCS2 or SIP18 mRNAs with PBs in response to 3 μM 1-NM-PP1.
  • Fig. S13. Modeling the relationship between the level of priming products and Hog1 adaptation time.
  • Fig. S14. Model fitting with all the experimental data.
  • Fig. S15. Computational modeling reproduces the modulation of memory dynamics in the absence of key regulatory factors.
  • Table S1. Strains constructed in this study.
  • Table S2. Initial conditions of all species in the model.
  • Table S3. Best-fit parameter values used in the model.

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