Supplementary Materials

The PDF file includes:

  • Text S1. Detailed HT-DBP protocol.
  • Fig. S1. Schematic of DBP.
  • Fig. S2. Selection of informative peptide screening concentrations.
  • Fig. S3. Identification of a drug screening concentration.
  • Fig. S4. HT-DBP enables identification of compounds that sensitize cancer cells for apoptosis.
  • Fig. S5. Similarity between FACS and microscopy dynamic BH3 profiles.
  • Fig. S6. Images of stained MMTV-PyMT tumor cells.
  • Fig. S7. Example of cell masks.
  • Fig. S8. Screening data for freshly isolated MMTV-PyMT tumors.
  • Fig. S9. Counterscreens in healthy cells.
  • Fig. S10. Supplemental data for in vivo validation experiments for HT-DBP.
  • Fig. S11. Identification of compounds that sensitize the COCA9 colon cancer PDX for apoptosis.
  • Fig. S12. Quantification of apoptotic chemical vulnerabilities in colon cancer PDX models.
  • Fig. S13. Heat map of apoptotic chemical vulnerabilities in colon cancer PDX models.
  • Fig. S14. Comparison of apoptotic chemical vulnerabilities in colon cancer PDX models.
  • Fig. S15. Supplemental data for comparison of chemical vulnerabilities of freshly isolated and cultured cancer cells.
  • Fig. S16. Supplemental data for comparison of chemical vulnerabilities of freshly isolated and cultured cancer cells.
  • Legend for table S1
  • Legends for data files S1 to S16

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Other Supplementary Material for this manuscript includes the following:

  • Table S1 (Microsoft Excel format). List of compounds used in the chemical screen.
  • Data file S1 (Microsoft Excel format). Cell count and delta priming of MDA-MB-231 line.
  • Data file S2 (Microsoft Excel format). Apoptosis and delta priming of MDA-MB-231 line.
  • Data file S3 (Microsoft Excel format). Cell count and delta priming of MMTV-PyMT tumor.
  • Data file S4 (Microsoft Excel format). Technical replicate of MMTV-PyMT delta priming.
  • Data file S5 (Microsoft Excel format). Biological replicate of MMTV-PyMT delta priming.
  • Data file S6 (Microsoft Excel format). Cell count and delta priming of primary mouse hepatocytes.
  • Data file S7 (Microsoft Excel format). Cell count and delta priming of human foreskin fibroblasts.
  • Data file S8 (Microsoft Excel format). Delta priming in adult mouse hepatocytes and MMTV-PyMT tumors.
  • Data file S9 (Microsoft Excel format). Delta priming of freshly isolated MMTV-PyMT tumors by nominal drug target.
  • Data file S10 (Microsoft Excel format). Normalized delta priming of colorectal PDX models.
  • Data file S11 (Microsoft Excel format). Normalized delta priming of colorectal PDX models by nominal target.
  • Data file S12 (Microsoft Excel format). Delta priming of MMTV-PyMT–derived cell line.
  • Data file S13 (Microsoft Excel format). Correlation between MMTV-PyMT tumor and derived cell line.
  • Data file S14 (Microsoft Excel format). Dose response by delta priming in MMTV-PyMT tumor and derived cell line.
  • Data file S15 (Microsoft Excel format). Delta priming in colorectal PDX models.
  • Data file S16 (Microsoft Excel format). Delta priming in primary human CRC cells ex vivo.