Supplementary Materials

This PDF file includes:

  • Fig. S1. The Smchd1 rhombohedral crystal structure used to solve the phase problem by single anomalous dispersion and electron density around Arg1762 in the tetragonal crystal structure.
  • Fig. S2. Coiled-coils flanking the core Smchd1 hinge domain are observed as two unassigned helices in the asymmetric unit, despite being present in intact MS analysis of SMCHD1 hinge dimer crystals.
  • Fig. S3. Crystal structures of SMC proteins with coiled-coils.
  • Fig. S4. Dimer interfaces of Smchd1 and canonical SMC hinge domains.
  • Fig. S5. Size exclusion chromatograms of Smchd1 hinge mutants.
  • Fig. S6. Tm values of WT and mutant Smchd1 hinge domains measured by DSF.
  • Fig. S7. The mutations R1848A and R1867G and deletion or compromise of hinge domain integrity alter the nuclear localization pattern of Smchd1.
  • Fig. S8. The mutations R1848A and R1867G perturb local interactions and abrogate formation of Smchd1 nuclear foci.
  • Table S1. Data collection and scattering parameters for SAXS analysis.

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