Supplementary Materials

The PDF file includes:

  • Fig. S1. RT-qPCR assay validation.
  • Fig. S2. The majority of LELs in intact cerebral arteries are immobile.
  • Fig. S3. Expression of SMC markers in proliferative SMCs.
  • Fig. S4. Validation of TRPML1, Lamp-1, and RyR2 primary antibodies used for super-resolution imaging.
  • Fig. S5. Localization accuracy of GSD super-resolution.
  • Fig. S6. Whole-cell BK currents and expression of Rry2, Kcnma1, and Kcnmb1 mRNAs in SMCs from Mcoln1−/− mice.
  • Fig. S7. Lack of spontaneous Ca2+-activated BK channel activity in proliferative SMCs.
  • Fig. S8. STOC amplitude after TRPML1 activation.
  • Fig. S9. Effect of vacuolar-type H+-ATPase (V-ATPase) inhibition on BK channel activity.
  • Fig. S10. STOC amplitude after TRPV4 activation.
  • Fig. S11. KCl-induced vasoconstriction.
  • Fig. S12. SNP-induced vasodilation.
  • Fig. S13. Heart rate and locomotor activity of WT and Mcoln1−/− mice.
  • Table S1. Nucleotide sequences of RT-qPCR primers.
  • Legends for movies S1 to S3

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.avi format). Representative recording of LysoTracker-stained particles in a native contractile SMC.
  • Movie S2 (.avi format). Representative recording of LysoTracker-stained particles in a pressurized cerebral artery.
  • Movie S3 (.avi format). Representative recording of LysoTracker-stained particles in a primary cultured proliferative SMC.