Supplementary Materials

The PDF file includes:

  • Fig. S1. Effect of BMP-1 on cell adhesion and apoptosis.
  • Fig. S2. BMP-1 alters cell adhesion through ECM modification.
  • Fig. S3. Characterization of TSP-1 fragments with various antibodies against the C-terminal and N-terminal domains of TSP-1.
  • Fig. S4. Mass spectrum and attribution of b and y ions for the peptide spanning residues 376 to 402 of human platelet TSP-1, defining BMP-1 cleavage site.
  • Fig. S5. Quantification of TSP-1 knockdown efficiency.
  • Fig. S6. Efficiency of coating 96-well plates with intact and cleaved TSP-1.
  • Fig. S7. SDS-PAGE analysis of purified fragment 1 and semipurified fragment 2 in nonreducing and reducing conditions.
  • Fig. S8. Alignment of TSP-1 from 12 species and human TSP-2 around the BMP-1 cleavage site.
  • Legends for movies S1 and S2
  • References (78, 79)

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Other Supplementary Material for this manuscript includes the following:

  • Movie S1 (.mp4 format). Time-lapse recording of subconfluent HT1080 cells.
  • Movie S2 (.mp4 format). Time-lapse recording of the morphological changes preceding cell detachment in subconfluent HT1080–BMP-1 cells.